The Human KISS1 (Kisspeptin 1) ELISA Kit is a specialized assay designed for the quantitative analysis of Kisspeptin 1 in human biological samples. Kisspeptin, a neuropeptide encoded by the KISS1 gene, plays a pivotal role in regulating reproductive function by stimulating the secretion of gonadotropin-releasing hormone (GnRH) and subsequent gonadotropin release, impacting fertility, puberty, and other reproductive processes. Our KISS1 ELISA Kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research or clinical studies. Manufactured under stringent quality control measures, this kit delivers reliable performance and ease of use, making it a reliable choice for investigating Kisspeptin 1 levels in various biological samples. Trust Assay Genie's KISS1 ELISA Kit for precise and dependable quantification of this important biomarker in your experimental endeavors.
Product Name:
Human KISS1 (Kisspeptin 1) ELISA Kit
SKU:
AEES00133
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
