The Human KL (Klotho) ELISA Kit is a highly reliable tool for the precise measurement of Klotho levels in human biological samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides accurate and consistent results, making it an excellent choice for various research applications.Klotho is a key protein that plays a critical role in regulating aging and age-related diseases by modulating multiple signaling pathways.
Dysregulation of Klotho has been linked to various health conditions, including kidney disease, cardiovascular disease, and cognitive decline. The Human KL ELISA Kit allows researchers to investigate the role of Klotho in these diseases and explore its potential as a therapeutic target.
Product Name:
Human KL (Klotho) ELISA Kit
SKU:
HUES03445
Target:
Human KL (Klotho)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KL. You can calculate the concentration of Human KL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
97-113
97-108
Average (%)
106
105
102
1:4
Range (%)
89-101
81-92
81-93
Average (%)
95
87
87
1:8
Range (%)
89-100
87-102
85-100
Average (%)
95
93
91
1:16
Range (%)
94-107
80-90
88-102
Average (%)
99
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.05
9.74
0.97
2.04
9.08
Standard deviation
0.06
0.11
0.34
0.05
0.1
0.48
C V (%)
5.77
5.37
3.49
5.15
4.9
5.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KL in samples. No significant cross-reactivity or interference between Human KL and analogues was observed.
