Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
The Human KRT10 (Keratin Type I Cytoskeletal 10) CLIA Kit is a reliable and accurate tool for measuring levels of KRT10 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research studies.KRT10 is a key protein involved in maintaining the structure and integrity of keratinocytes in the skin.
Changes in KRT10 levels have been associated with various skin disorders, making it a valuable biomarker for studying skin diseases and developing therapeutic interventions.Overall, the Human KRT10 CLIA Kit is essential for researchers in the fields of dermatology, cell biology, and molecular biology who are interested in studying the role of KRT10 in skin health and disease.
Product Name:
Human KRT10 (Keratin, Type I Cytoskeletal 10) CLIA Kit
SKU:
HUES01129
Target:
Human KRT10 (Keratin, Type I Cytoskeletal 10)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRT10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRT10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human KRT10. You can calculate the concentration of Human KRT10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-113
94-110
88-102
Average (%)
107
101
95
1:4
Range (%)
101-113
86-99
96-111
Average (%)
107
91
102
1:8
Range (%)
88-102
84-96
96-113
Average (%)
93
90
104
1:16
Range (%)
90-103
97-111
98-115
Average (%)
97
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-108
102
EDTA plasma (n=5)
88-103
96
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
27.48
65.37
171.25
27.44
67.27
165.6
Standard deviation
2.7
6.92
10.28
3.36
5.11
12.04
C V (%)
9.83
10.59
6.0
12.24
7.6
7.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human KRT10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed.
