Human KRT10 (Keratin, Type I Cytoskeletal 10) ELISA Kit
The Human KRT10 (Keratin, Type I Cytoskeletal 10) ELISA Kit is specifically designed to quantitatively measure the levels of KRT10 in human biological samples.
Keratins are essential structural proteins that provide integrity and resistance to mechanical stress in epithelial cells, including skin, hair, and nails. KRT10, a type I cytokeratin, plays a crucial role in maintaining the cytoskeletal architecture and integrity of epithelial tissues. Our KRT10 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results in your research or clinical investigations. With stringent quality control standards, this kit delivers robust and reliable performance for precise quantification of KRT10 levels. Trust Assay Genie's KRT10 ELISA Kit for a user-friendly experience and dependable quantification of this key biomarker in your studies.
Product Name:
Human KRT10 (Keratin, Type I Cytoskeletal 10) ELISA Kit
SKU:
AEES00132
Target:
Human KRT10 (Keratin, Type I Cytoskeletal 10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KRT10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRT10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRT10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KRT10. You can calculate the concentration of Human KRT10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-99
101-109
95-109
Average (%)
95
105
103
1:4
Range (%)
88-97
84-94
94-103
Average (%)
94
90
97
1:8
Range (%)
93-103
84-97
88-96
Average (%)
98
90
93
1:16
Range (%)
100-109
89-101
89-96
Average (%)
103
97
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-95
92
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
100-107
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.59
106.12
433.09
46.7
103.75
435.89
Standard deviation
2.7
5.31
21.65
2.65
4.83
19.27
C V (%)
5.67
5.0
5.0
5.67
4.66
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KRT10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KRT10 in samples. No significant cross-reactivity or interference between Human KRT10 and analogues was observed.
