Human LDLR (Low Density Lipoprotein Receptor) CLIA Kit
The Human LDLR (Low-Density Lipoprotein Receptor) CLIA Kit is designed for the precise measurement of LDLR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The LDLR protein is a key player in cholesterol metabolism, facilitating the uptake of LDL cholesterol from the bloodstream into cells. Dysregulation of LDLR function is associated with atherosclerosis, hypercholesterolemia, and other cardiovascular diseases.
Therefore, measuring LDLR levels can provide valuable insights into these conditions and aid in the development of potential treatments.The Human LDLR CLIA Kit from Assay Genie is a valuable tool for researchers studying lipid metabolism, cardiovascular health, and related diseases. Its reliability and precision make it an essential component of any research project focused on understanding the role of LDLR in human health and disease.
Product Name:
Human LDLR (Low Density Lipoprotein Receptor) CLIA Kit
SKU:
HUES00692
Target:
Human LDLR (Low Density Lipoprotein Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LDLR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDLR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDLR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LDLR. You can calculate the concentration of Human LDLR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
91-105
97-110
Average (%)
100
96
104
1:4
Range (%)
100-118
88-99
96-107
Average (%)
108
93
101
1:8
Range (%)
90-104
88-104
97-113
Average (%)
96
95
104
1:16
Range (%)
94-108
90-105
101-114
Average (%)
101
97
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
189.01
335.31
1502.02
205.6
310.76
1626.23
Standard deviation
20.79
26.15
146.15
20.17
36.55
153.84
C V (%)
11.0
7.8
9.73
9.81
11.76
9.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LDLR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LDLR in samples. No significant cross-reactivity or interference between Human LDLR and analogues was observed.
