Based on the information provided, we have the Human LEPR (Leptin Receptor) CLIA Kit, designed for accurate detection of leptin receptor levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity for reliable and reproducible results, making it versatile for various research applications.The leptin receptor is a key component in the regulation of energy balance and metabolism, playing a crucial role in obesity, diabetes, and other metabolic disorders.
It is also implicated in various autoimmune and inflammatory diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.Overall, the Human LEPR CLIA Kit from Assay Genie provides researchers with a powerful tool to study the role of leptin receptor in health and disease, offering accurate and precise measurements to advance scientific discoveries.
Product Name:
Human LEPR (Leptin Receptor) CLIA Kit
SKU:
HUES00089
Target:
Human LEPR (Leptin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LEPR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LEPR. You can calculate the concentration of Human LEPR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-115
102-120
86-98
Average (%)
109
110
91
1:4
Range (%)
101-119
95-108
91-102
Average (%)
109
100
97
1:8
Range (%)
99-115
86-98
94-106
Average (%)
105
92
99
1:16
Range (%)
87-102
86-101
102-118
Average (%)
93
93
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-112
103
EDTA plasma (n=5)
98-114
105
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
250.13
716.79
2092.88
271.52
657.86
2086.95
Standard deviation
23.71
67.95
156.55
33.37
48.16
218.29
C V (%)
9.48
9.48
7.48
12.29
7.32
10.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LEPR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LEPR in samples. No significant cross-reactivity or interference between Human LEPR and analogues was observed.
