The Human LH (Luteinizing Hormone) CLIA Kit is specifically designed for the precise measurement of LH levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and consistent results for various research purposes.Luteinizing Hormone is a vital hormone that plays a key role in the reproductive system, particularly in the regulation of ovulation and the production of sex hormones. Abnormal levels of LH can indicate fertility issues and hormonal imbalances, making it a valuable marker for studying reproductive health and developing interventions for related conditions.
Overall, the Human LH CLIA Kit is an essential tool for researchers and clinicians working in the fields of endocrinology, fertility, and reproductive health. Its reliability and precision make it a valuable asset for advancing scientific knowledge and improving patient care.
Product Name:
Human LH (Luteinizing Hormone) CLIA Kit
SKU:
HUES00111
Target:
Human LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 mIU/mL
Detection range:
0.31-20 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LH. You can calculate the concentration of Human LH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
85-99
89-103
Average (%)
101
92
96
1:4
Range (%)
90-104
85-96
86-98
Average (%)
97
90
93
1:8
Range (%)
102-116
92-108
100-116
Average (%)
109
99
106
1:16
Range (%)
95-110
88-101
94-108
Average (%)
100
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
99
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.92
8.15
1.05
3.0
8.2
Standard deviation
0.12
0.27
0.54
0.11
0.25
0.56
C V (%)
12.24
9.25
6.63
10.48
8.33
6.83
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LH in samples. No significant cross-reactivity or interference between Human LH and analogues was observed.
