The LH (Luteinizing Hormone) ELISA Kit is meticulously developed for the quantitative analysis of Luteinizing Hormone levels in various human samples. Luteinizing Hormone, a critical hormone involved in reproductive function, specifically regulating ovulation and estrogen production in females and testosterone production in males. Accurate measurement of LH is essential for assessing fertility, diagnosing reproductive disorders, and monitoring hormonal imbalances.
This ELISA kit ensures exceptional sensitivity and specificity, providing reliable and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust performance and is user-friendly, making it ideal for both research and clinical applications. Rely on Assay Genie's LH ELISA Kit for accurate and dependable quantification of this vital hormone in your studies.
Product Name:
Human LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00055
Target:
Human LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 mIU/mL
Detection range:
0.16-10 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LH. You can calculate the concentration of Human LH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
90-105
87-97
Average (%)
91
97
93
1:4
Range (%)
94-102
83-97
97-110
Average (%)
97
90
105
1:8
Range (%)
87-96
88-97
96-109
Average (%)
91
91
103
1:16
Range (%)
90-99
89-100
88-95
Average (%)
94
95
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
90-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.2
4.13
0.45
1.16
4.91
Standard deviation
0.03
0.05
0.23
0.02
0.06
0.25
C V (%)
6.12
4.17
5.57
4.44
5.17
5.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LH in samples. No significant cross-reactivity or interference between Human LH and analogues was observed.
