Human LOXL4 (Lysyl Oxidase Like Protein 4) CLIA Kit
The Human LOXL4 (Lysyl Oxidase-Like Protein 4) CLIA Kit is a cutting-edge assay designed for the precise measurement of LOXL4 levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for a variety of research applications.LOXL4 is a key enzyme belonging to the lysyl oxidase family, involved in the crosslinking of collagen and elastin fibers in the extracellular matrix. Dysregulation of LOXL4 has been implicated in various diseases, including cancer, fibrosis, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development.
By utilizing the Human LOXL4 CLIA Kit, researchers can gain insights into the role of LOXL4 in pathophysiological processes and explore its therapeutic potential in addressing various health conditions. Invest in this innovative assay today to advance your research efforts and uncover novel insights into LOXL4 biology.
Product Name:
Human LOXL4 (Lysyl Oxidase Like Protein 4) CLIA Kit
SKU:
HUES01273
Target:
Human LOXL4 (Lysyl Oxidase Like Protein 4 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LOXL4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LOXL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LOXL4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LOXL4. You can calculate the concentration of Human LOXL4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
102-118
100-114
Average (%)
98
109
106
1:4
Range (%)
87-101
91-102
99-111
Average (%)
92
96
105
1:8
Range (%)
93-106
90-105
89-99
Average (%)
99
97
94
1:16
Range (%)
85-97
93-105
98-110
Average (%)
91
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
91-106
97
Cell culture media (n=5)
102-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
206.0
440.71
1829.87
222.42
418.65
1930.02
Standard deviation
16.87
46.63
118.58
22.69
39.06
196.09
C V (%)
8.19
10.58
6.48
10.2
9.33
10.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LOXL4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LOXL4 in samples. No significant cross-reactivity or interference between Human LOXL4 and analogues was observed.
