Human Lymphocyte Function Associated Antigen 3 (LFA3) ELISA Kit
The Human Lymphocyte Function-Associated Antigen 3 (LFA3) ELISA Kit is specifically designed for the accurate measurement of LFA3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.LFA3 is a critical protein involved in the regulation of lymphocyte function and immune response. It plays a key role in the interaction between T cells and antigen-presenting cells, influencing the activation and proliferation of immune cells.
The detection of LFA3 levels can provide valuable insights into the function of lymphocytes and their role in immune regulation.This ELISA kit is essential for researchers studying immune system function, inflammation, autoimmune diseases, and cancer. By accurately measuring LFA3 levels, researchers can better understand the mechanisms underlying immune responses and develop targeted therapies for various disease conditions. Trust in the Human Lymphocyte Function-Associated Antigen 3 (LFA3) ELISA Kit for precise and reliable results in your research endeavors.
Product Name:
Human Lymphocyte Function Associated Antigen 3 (LFA3) ELISA Kit
Product Code:
HUDL01710
Size:
96 Assays
Target:
LFA3
Synonyms:
CD58, Ag3, Surface glycoprotein LFA-3
Detection Method:
Sandwich
Range:
12.5-800pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
