The Human LYZ (Lysozyme) CLIA Kit is a powerful tool for the precise quantification of lysozyme levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Lysozyme, a key antimicrobial enzyme, plays a critical role in the innate immune system by breaking down bacterial cell walls.
Its levels can be indicative of inflammatory processes, infections, and other immune-related conditions, making it a valuable biomarker for studying immune responses and developing novel therapeutic approaches.With its user-friendly protocol and robust performance, the Human LYZ (Lysozyme) CLIA Kit is an invaluable resource for researchers interested in investigating the role of lysozyme in health and disease.
Product Name:
Human LZM (Lysozyme) CLIA Kit
SKU:
HUES01034
Target:
Human LZM (Lysozyme)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LZM. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LZM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LZM. You can calculate the concentration of Human LZM in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
97-109
90-101
Average (%)
104
103
96
1:4
Range (%)
84-97
93-109
83-95
Average (%)
91
101
90
1:8
Range (%)
91-104
103-121
86-96
Average (%)
98
110
91
1:16
Range (%)
89-104
103-118
96-108
Average (%)
95
110
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
104
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.35
6.7
0.93
2.44
7.11
Standard deviation
0.1
0.25
0.69
0.12
0.19
0.59
C V (%)
10.75
10.64
10.3
12.9
7.79
8.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LZM concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LZM in samples. No significant cross-reactivity or interference between Human LZM and analogues was observed.
