Human Macrophage Derived Chemokine (MDC) ELISA Kit
The Human Macrophage-Derived Chemokine (MDC) ELISA Kit is specifically designed for the precise detection of MDC levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.MDC is a key chemokine involved in the immune response, playing a crucial role in the recruitment and activation of immune cells. Elevated levels of MDC have been associated with various inflammatory diseases, making it a pivotal biomarker for studying and potentially treating these conditions.
With its advanced technology and reliable performance, the Human MDC ELISA Kit is an indispensable tool for researchers looking to explore the role of MDC in immune responses and inflammatory disorders. Get accurate and reliable results with this innovative ELISA kit from Assay Genie.
Product Name:
Human Macrophage Derived Chemokine (MDC) ELISA Kit
Product Code:
HUDL01821
Size:
96 Assays
Target:
MDC
Synonyms:
CCL22, ABCD1, DC/B-CK, SCYA22, STCP1, Chemokine C-C-Motif Ligand 22, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22, Stimulated T-Cell Chemotactic Protein 1
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
