Human MDC (Macrophage Derived Chemokine) ELISA Kit
The Human MDC (Macrophage-Derived Chemokine) ELISA Kit is specifically designed for the precise detection of MDC levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for various research applications.MDC is a key chemokine involved in recruiting immune cells to sites of inflammation and participating in the regulation of immune responses. Its levels have been implicated in various inflammatory conditions, autoimmune diseases, and cancer, highlighting its importance as a biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human MDC ELISA Kit from AssayGenie provides researchers with a reliable and efficient method for quantifying MDC levels, advancing our understanding of immune responses and inflammation-related diseases.
Product Name:
Human MDC (Macrophage Derived Chemokine) ELISA Kit
SKU:
HUES01316
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
