Human MAG (Myelin Associated Glycoprotein) CLIA Kit
According to the information provided at www.assaygenie.com/human-mag-myelin-associated-glycoprotein-clia-kit-hues00243/, the Human MAG (Myelin-Associated Glycoprotein) CLIA Kit is specifically designed for the accurate detection of MAG levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Myelin-Associated Glycoprotein (MAG) is a key protein involved in the formation and maintenance of myelin, a crucial component of the nervous system.
Dysregulation of MAG has been linked to various neurological disorders such as multiple sclerosis, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.Overall, the Human MAG CLIA Kit provides researchers with a powerful tool for studying the role of MAG in neurological diseases and advancing our understanding of the underlying mechanisms involved.
Product Name:
Human MAG (Myelin Associated Glycoprotein) CLIA Kit
SKU:
HUES00243
Target:
Human MAG (Myelin Associated Glycoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MAG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MAG. You can calculate the concentration of Human MAG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
102-116
99-109
Average (%)
100
110
104
1:4
Range (%)
102-114
93-108
101-113
Average (%)
108
101
107
1:8
Range (%)
89-101
94-111
88-100
Average (%)
95
102
93
1:16
Range (%)
90-100
87-99
100-116
Average (%)
95
92
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
213.83
341.14
1979.58
193.99
369.09
2096.04
Standard deviation
21.7
25.89
217.95
24.33
36.69
206.88
C V (%)
10.15
7.59
11.01
12.54
9.94
9.87
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MAG concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human MAG in samples. No significant cross-reactivity or interference between Human MAG and analogues was observed.
