Human Major Histocompatibility Complex Class I G (MHCG) ELISA Kit
The Human Major Histocompatibility Complex Class I (MHCG) ELISA Kit is specifically designed for the precise and reliable detection of MHCG levels in human samples including serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The Major Histocompatibility Complex Class I proteins play a critical role in immune response by presenting antigens to T cells, thereby regulating the body's immune system. Dysregulation of MHCG expression has been implicated in various diseases including autoimmune disorders, viral infections, and cancer, highlighting its importance as a potential biomarker for disease progression and therapy development.
With the Human Major Histocompatibility Complex Class I (MHCG) ELISA Kit, researchers can confidently study and analyze MHCG levels in human samples, providing valuable insights into immune response mechanisms and disease pathology. Choose this kit for its superior performance and reliability in advancing your research efforts.
Product Name:
Human Major Histocompatibility Complex Class I G (MHCG) ELISA Kit
Product Code:
HUDL01850
Size:
96 Assays
Target:
MHCG
Synonyms:
HLA-G, MHC-G, HLAG, Leukocyte Antigen G, b2 microglobulin, HLA class I histocompatibility antigen, alpha chain G
Detection Method:
Sandwich
Range:
0.375-24ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
