Human MAPtau (Microtubule Associated Protein Tau/Tau Protein) CLIA Kit
The Human MAPTAU (Microtubule-Associated Protein Tau) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of tau protein levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications in the field of neurodegenerative disorders.Tau protein is a key player in the pathogenesis of neurodegenerative diseases such as Alzheimer's disease, where abnormal tau aggregation leads to the formation of neurofibrillary tangles.
Studying tau protein levels can provide valuable insights into the progression of these conditions and aid in the development of potential therapeutic interventions.With its high sensitivity and specificity, the Human MAPTAU CLIA Kit from AssayGenie is a valuable tool for researchers looking to accurately measure tau protein levels and unravel the complexities of neurodegenerative disorders.
Product Name:
Human MAPtau (Microtubule Associated Protein Tau/Tau Protein) CLIA Kit
SKU:
HUES00564
Target:
Human MAPtau (Microtubule Associated Protein Tau/Tau Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MAPÏ„. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAPÏ„ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAPÏ„, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MAPÏ„. You can calculate the concentration of Human MAPÏ„ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
94-110
98-114
Average (%)
94
102
106
1:4
Range (%)
87-99
88-99
86-100
Average (%)
93
94
93
1:8
Range (%)
86-100
83-96
96-108
Average (%)
91
90
102
1:16
Range (%)
88-103
99-114
93-107
Average (%)
94
106
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
18.97
38.93
154.43
20.23
42.32
156.66
Standard deviation
2.36
3.99
14.18
2.27
4.63
9.42
C V (%)
12.44
10.25
9.18
11.22
10.94
6.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MAPÏ„ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MAPÏ„ in samples. No significant cross-reactivity or interference between Human MAPÏ„ and analogues was observed.
