The Human Matrix Metalloproteinase-2 (MMP2) ELISA Kit is a cutting-edge tool designed for precise measurement of MMP2 levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable asset for various research purposes.Matrix Metalloproteinase-2 (MMP2) is a key enzyme involved in extracellular matrix degradation, cell migration, and tissue remodeling. Its dysregulation has been linked to numerous pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders.
Therefore, the MMP2 ELISA Kit serves as a valuable resource for investigating these conditions and potentially developing targeted therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human Matrix Metalloproteinase-2 (MMP2) ELISA Kit from Assay Genie. Order yours today and unlock new insights into the role of MMP2 in health and disease.
Product Name:
Human Matrix Metalloproteinase 2 (MMP2) ELISA Kit
Product Code:
HUDL01883
Size:
96 Assays
Target:
MMP2
Synonyms:
CLG4, CLG4A, MMP-II, MONA, TBE-1, PEX, Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
