The Human MAU (Microalbuminuria) CLIA Kit is specifically designed for the precise measurement of microalbuminuria levels in human samples, including serum, plasma, and urine. This kit offers exceptional sensitivity and specificity, ensuring the accuracy and reproducibility of results for various research applications.Microalbuminuria is a key indicator of renal dysfunction and cardiovascular risk, making it a valuable biomarker for monitoring and diagnosing conditions such as chronic kidney disease, diabetes, and hypertension.
By accurately measuring microalbuminuria levels, researchers can gain insights into disease progression and treatment effectiveness, ultimately leading to improved patient outcomes.
Product Name:
Human MAU (Microalbuminuria) CLIA Kit
SKU:
HUES00110
Target:
Human MAU (Microalbuminuria)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 μg/mL
Detection range:
0.63-40 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MAU. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MAU. You can calculate the concentration of Human MAU in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
100-113
101-115
Average (%)
93
106
108
1:4
Range (%)
87-102
99-110
101-114
Average (%)
94
104
108
1:8
Range (%)
95-111
88-102
87-99
Average (%)
101
96
92
1:16
Range (%)
87-100
101-118
92-107
Average (%)
94
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
92
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.84
5.63
18.37
1.75
5.34
16.76
Standard deviation
0.2
0.58
1.2
0.22
0.41
1.83
C V (%)
10.87
10.3
6.53
12.57
7.68
10.92
Sample type &Sample volume:
urine; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MAU concentrations in urine.
Specificity:
This kit recognizes Human MAU in samples. No significant cross-reactivity or interference between Human MAU and analogues was observed.
