Human MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
The Human MCP-1 (Monocyte Chemoattractant Protein-1) CLIA Kit is a reliable and accurate tool for the quantitative detection of MCP-1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications.MCP-1 is a key chemokine involved in the recruitment of monocytes to sites of inflammation and tissue injury. Elevated levels of MCP-1 have been implicated in numerous inflammatory conditions, such as atherosclerosis, arthritis, and diabetes.
By accurately measuring MCP-1 levels, researchers can gain valuable insights into the pathogenesis of these diseases and identify potential therapeutic targets.With its robust performance and ease of use, the Human MCP-1 CLIA Kit is an indispensable tool for researchers studying inflammation, immune response, and related diseases. Order now and accelerate your research with confidence.
Product Name:
Human MCP-1 (Monocyte Chemotactic Protein 1) CLIA Kit
SKU:
HUES00020
Target:
Human MCP-1 (Monocyte Chemotactic Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP-1. You can calculate the concentration of Human MCP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
88-102
88-99
Average (%)
102
93
94
1:4
Range (%)
91-106
90-105
93-111
Average (%)
97
96
101
1:8
Range (%)
93-106
102-115
101-114
Average (%)
99
109
108
1:16
Range (%)
84-96
96-108
97-114
Average (%)
91
102
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-112
106
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.97
50.59
231.9
24.71
45.93
218.62
Standard deviation
3.42
4.44
20.06
2.94
3.69
22.06
C V (%)
12.68
8.78
8.65
11.9
8.03
10.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP-1 in samples. No significant cross-reactivity or interference between Human MCP-1 and analogues was observed.
