Human MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
The Human MCP-3 (Monocyte Chemotactic Protein-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MCP-3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a wide range of research applications.MCP-3 is a key chemokine involved in the recruitment and activation of monocytes, playing a crucial role in inflammatory processes and immune response.
Abnormal levels of MCP-3 have been implicated in various inflammatory conditions, making it an important biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human MCP-3 CLIA Kit is a valuable tool for researchers investigating the role of MCP-3 in inflammation, immune response, and disease pathogenesis. Its high sensitivity and specificity ensure accurate and reliable measurements for meaningful research outcomes.
Product Name:
Human MCP-3 (Monocyte Chemotactic Protein 3) CLIA Kit
SKU:
HUES00024
Target:
Human MCP-3 (Monocyte Chemotactic Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCP-3. You can calculate the concentration of Human MCP-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-117
98-111
88-99
Average (%)
108
105
93
1:4
Range (%)
97-112
94-107
90-102
Average (%)
103
100
95
1:8
Range (%)
98-114
89-104
101-116
Average (%)
106
95
107
1:16
Range (%)
88-101
89-99
86-97
Average (%)
94
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
93-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.3
53.26
234.51
26.68
54.06
254.4
Standard deviation
3.14
5.48
16.51
2.24
4.0
17.43
C V (%)
12.41
10.29
7.04
8.4
7.4
6.85
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCP-3 in samples. No significant cross-reactivity or interference between Human MCP-3 and analogues was observed.
