Human MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
The Human MCP-3 (Monocyte Chemotactic Protein-3) ELISA Kit is specially designed for the precise measurement of MCP-3 levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MCP-3 is a key chemokine involved in the recruitment of monocytes to sites of inflammation, playing a crucial role in immune response regulation. Dysregulation of MCP-3 has been linked to various inflammatory conditions, autoimmune diseases, and cancer progression, making it a valuable biomarker for investigating these diseases and potential therapeutic interventions. With its advanced technology and reliable performance, the Human MCP-3 ELISA Kit is an indispensable tool for researchers seeking to study the role of MCP-3 in health and disease, ultimately advancing our understanding and treatment of inflammatory disorders.
Product Name:
Human MCP-3 (Monocyte Chemotactic Protein 3) ELISA Kit
SKU:
HUES01311
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
