Human MCSF (Macrophage Colony Stimulating Factor 1) CLIA Kit
The Human MCSF (Macrophage Colony-Stimulating Factor) CLIA Kit is a powerful tool for the quantitative measurement of MCSF levels in human biological samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.MCSF is a key cytokine that plays a vital role in the differentiation and maturation of macrophages, important immune cells that are involved in various physiological and pathological processes.
Dysregulation of MCSF levels has been implicated in conditions such as inflammation, autoimmune diseases, and cancer, highlighting the significance of studying this cytokine as a potential biomarker for disease diagnosis and therapeutic development.With the Human MCSF CLIA Kit, researchers can gain valuable insights into the role of MCSF in health and disease, paving the way for potential new treatments and improved patient outcomes.
Product Name:
Human MCSF (Macrophage Colony Stimulating Factor 1) CLIA Kit
SKU:
HUES00093
Target:
Human MCSF (Macrophage Colony Stimulating Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MCSF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MCSF. You can calculate the concentration of Human MCSF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-110
99-115
88-104
Average (%)
101
105
95
1:4
Range (%)
84-99
93-104
87-99
Average (%)
91
99
93
1:8
Range (%)
87-102
98-110
102-117
Average (%)
93
104
109
1:16
Range (%)
93-105
92-105
89-103
Average (%)
99
98
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.28
71.87
375.9
39.45
68.71
338.75
Standard deviation
4.82
7.7
41.54
3.45
7.72
30.32
C V (%)
12.93
10.71
11.05
8.75
11.24
8.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MCSF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MCSF in samples. No significant cross-reactivity or interference between Human MCSF and analogues was observed.
