Human Membrane Protein, Palmitoylated 6 (MPP6) ELISA Kit
The Human Membrane Protein Palmitoylated 6 (MPP6) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of MPP6 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.MPP6 is a membrane protein that plays a crucial role in cellular functions such as signal transduction, cell adhesion, and cell-cell communication. Dysregulation of MPP6 has been linked to various diseases, making it an important biomarker for studying these conditions and potentially developing new treatments.
With its high sensitivity and specificity, the Human Membrane Protein Palmitoylated 6 (MPP6) ELISA Kit is a valuable tool for researchers looking to explore the role of MPP6 in health and disease. Order now to enhance your research capabilities and gain valuable insights into the functions of this important membrane protein.
Product Name:
Human Membrane Protein, Palmitoylated 6 (MPP6) ELISA Kit
Product Code:
HUDL01899
Size:
96 Assays
Target:
MPP6
Synonyms:
PALS2, VAM1, P55T, MAGUK P55 Subfamily Member 6, Veli-associated MAGUK 1
Detection Method:
Sandwich
Range:
12.5-800pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
