Human MHCG (Major Histocompatibility Complex Class I G) CLIA Kit
The Human MHC-I (Major Histocompatibility Complex Class I) ELISA Kit is specifically designed for the quantitative detection of MHC-I levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.MHC-I molecules play a key role in the immune system by presenting antigens to T cells, helping to regulate immune responses and defend against infections.
Dysregulation of MHC-I expression is associated with autoimmune diseases, viral infections, and cancer, making it an important biomarker for studying these conditions and developing potential therapies.Overall, the Human MHC-I ELISA Kit provides researchers with a valuable tool for studying the role of MHC-I in immune responses and disease pathogenesis, with the reliability and performance needed for in-depth research and analysis.
Product Name:
Human MHCG (Major Histocompatibility Complex Class I G) CLIA Kit
SKU:
HUES00936
Target:
Human MHCG (Major Histocompatibility Complex Class I G)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MHCG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MHCG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MHCG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MHCG. You can calculate the concentration of Human MHCG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
89-104
89-102
Average (%)
110
96
96
1:4
Range (%)
94-109
100-112
85-99
Average (%)
102
107
92
1:8
Range (%)
96-108
98-111
89-101
Average (%)
102
104
95
1:16
Range (%)
97-113
85-98
90-103
Average (%)
105
91
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
100-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.59
3.46
0.47
1.65
3.43
Standard deviation
0.04
0.17
0.34
0.05
0.13
0.25
C V (%)
8.16
10.69
9.83
10.64
7.88
7.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MHCG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MHCG in samples. No significant cross-reactivity or interference between Human MHCG and analogues was observed.
