Human MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
The Human MIP-1 Alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit is specifically designed for the accurate detection of MIP-1 Alpha levels in human samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.MIP-1 Alpha is a key chemokine involved in the immune response, particularly in attracting and activating immune cells such as macrophages and T cells. Its role in inflammation and immune regulation makes it a valuable biomarker for studying immune-related diseases, infectious diseases, and inflammatory conditions.
With the Human MIP-1 Alpha CLIA Kit, researchers can uncover important insights into the role of MIP-1 Alpha in various physiological and pathological processes, ultimately leading to the development of potential therapeutic strategies. Get accurate and meaningful results with this advanced CLIA Kit from Assay Genie.
Product Name:
Human MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha) CLIA Kit
SKU:
HUES00021
Target:
Human MIP-1 alpha (Macrophage Inflammatory Protein 1 Alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIP-1α. You can calculate the concentration of Human MIP-1α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
93-107
102-120
Average (%)
94
101
110
1:4
Range (%)
100-116
90-102
99-115
Average (%)
106
96
106
1:8
Range (%)
89-101
87-100
88-103
Average (%)
95
93
94
1:16
Range (%)
85-96
87-98
87-101
Average (%)
91
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
97-111
104
Cell culture media (n=5)
100-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.2
107.86
347.92
39.81
115.66
358.19
Standard deviation
3.39
9.71
31.52
4.94
13.61
24.29
C V (%)
8.65
9.0
9.06
12.41
11.77
6.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIP-1α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP-1α in samples. No significant cross-reactivity or interference between Human MIP-1α and analogues was observed.
