Human MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
The Human MIP-3 Alpha (Macrophage Inflammatory Protein-3 Alpha) CLIA Kit is a cutting-edge assay designed for the precise quantification of MIP-3 Alpha levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.MIP-3 Alpha is a vital chemokine that plays a crucial role in immune response regulation, particularly in the recruitment of immune cells to sites of inflammation.
Dysregulation of MIP-3 Alpha has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.With its advanced technology and reliable performance, the Human MIP-3 Alpha CLIA Kit is an indispensable tool for researchers and clinicians looking to deepen their understanding of immune responses and inflammatory processes.
Product Name:
Human MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) CLIA Kit
SKU:
HUES00027
Target:
Human MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-3α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-3α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-3α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIP-3α. You can calculate the concentration of Human MIP-3α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-109
101-115
89-102
Average (%)
99
108
97
1:4
Range (%)
93-108
90-103
95-107
Average (%)
100
96
101
1:8
Range (%)
98-114
93-111
95-108
Average (%)
105
101
101
1:16
Range (%)
84-98
92-104
99-111
Average (%)
90
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
93
EDTA plasma (n=5)
85-96
91
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.32
57.87
223.32
24.56
57.95
222.84
Standard deviation
2.15
4.35
14.14
2.95
5.2
16.31
C V (%)
8.84
7.52
6.33
12.01
8.97
7.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIP-3α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP-3α in samples. No significant cross-reactivity or interference between Human MIP-3α and analogues was observed.
