Human MIP-3beta/ELC/CCL19 (Macrophage Inflammatory Protein 3beta) ELISA Kit
The Human MIP-3β/ELC/CCL19 (Macrophage Inflammatory Protein 3β) ELISA Kit is meticulously crafted to enable the precise quantitative detection of Macrophage Inflammatory Protein 3β levels in diverse biological samples. Macrophage Inflammatory Protein 3β, a chemokine known for its role in regulating immune responses, particularly in the recruitment of immune cells to sites of inflammation, is crucial in various physiological and pathological processes. Accurate measurement of Macrophage Inflammatory Protein 3β levels provides valuable insights into immune system modulation, inflammatory responses, and immune cell migration within tissues.
This ELISA kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Designed and manufactured under stringent quality control standards, the kit delivers reliable performance and user-friendly protocols, making it ideal for both research studies and clinical applications. Count on the Human MIP-3β/ELC/CCL19 ELISA Kit for precise and consistent quantitation of this pivotal biomarker in your investigations.
Product Name:
Human MIP-3beta/ELC/CCL19 (Macrophage Inflammatory Protein 3beta) ELISA Kit
SKU:
AEES00115
Target:
Human MIP-3β/ELC/CCL19 (Macrophage Inflammatory Protein 3β)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-3β/ELC/CCL19. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-3β/ELC/CCL19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-3β/ELC/CCL19, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MIP-3β/ELC/CCL19. You can calculate the concentration of Human MIP-3β/ELC/CCL19 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-105
98-112
90-98
Average (%)
100
104
95
1:4
Range (%)
92-103
84-98
92-107
Average (%)
96
90
100
1:8
Range (%)
88-98
87-95
96-107
Average (%)
91
91
104
1:16
Range (%)
98-109
91-102
86-95
Average (%)
101
98
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
95
EDTA plasma (n=5)
94-104
99
Cell culture media (n=5)
94-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.52
53.02
228.89
22.8
62.03
249.79
Standard deviation
1.21
2.57
9.29
1.22
2.84
10.12
C V (%)
5.37
4.85
4.06
5.35
4.58
4.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MIP-3β/ELC/CCL19 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP-3β/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3β/ELC/CCL19 and analogues was observed.
