Human MIP4 alpha (Macrophage Inflammatory Protein 4 Alpha) CLIA Kit
The Human MIP-4 (Macrophage Inflammatory Protein 4) CLIA Kit is specifically designed for the accurate detection of MIP-4 alpha levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for various research applications.MIP-4 alpha is a key chemokine involved in the recruitment and activation of monocytes and macrophages, playing a crucial role in inflammation and immune responses.
Dysregulation of MIP-4 alpha has been associated with various inflammatory diseases, making it an important biomarker for studying these conditions and developing potential therapeutic interventions.With the Human MIP-4 alpha CLIA Kit, researchers can easily and efficiently measure MIP-4 alpha levels, providing valuable insights into the mechanisms of inflammation and immune regulation. Trust in this kit for reliable and accurate results in your research endeavors.
Product Name:
Human MIP4 alpha (Macrophage Inflammatory Protein 4 Alpha) CLIA Kit
SKU:
HUES00031
Target:
Human MIP4 alpha (Macrophage Inflammatory Protein 4 Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIP4α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP4α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP4α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIP4α. You can calculate the concentration of Human MIP4α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
91-105
89-100
Average (%)
100
97
94
1:4
Range (%)
99-115
86-97
95-110
Average (%)
106
92
101
1:8
Range (%)
103-119
88-103
83-99
Average (%)
109
95
90
1:16
Range (%)
92-106
87-97
99-114
Average (%)
97
92
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
103
EDTA plasma (n=5)
99-117
106
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.88
91.75
382.74
43.8
96.24
368.42
Standard deviation
4.95
7.32
30.58
5.46
8.88
42.26
C V (%)
10.34
7.98
7.99
12.47
9.23
11.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIP4α concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP4α in samples. No significant cross-reactivity or interference between Human MIP4α and analogues was observed.
