Human Mitogen Activated Protein Kinase 13 (MAPK13) ELISA Kit
The Human Mitogen-Activated Protein Kinase 13 (MAPK13) ELISA Kit is a powerful tool for the accurate measurement of MAPK13 levels in human samples, including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit provides researchers with reliable and reproducible results for a variety of research applications.MAPK13 is a key player in cell signaling pathways, regulating important cellular processes such as proliferation, differentiation, and cell survival.
Dysregulation of MAPK13 has been linked to various diseases, including cancer, inflammatory disorders, and cardiovascular diseases. By measuring MAPK13 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human MAPK13 ELISA Kit is a valuable tool for researchers aiming to study the role of MAPK13 in health and disease, offering accurate and precise measurements for insightful research outcomes.
Product Name:
Human Mitogen Activated Protein Kinase 13 (MAPK13) ELISA Kit
Product Code:
HUDL01792
Size:
96 Assays
Target:
MAPK13
Synonyms:
SAPK4, p38 Delta, Mitogen-activated protein kinase p38 delta, Stress-activated protein kinase 4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
