Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
The Human MMP-12 (Matrix Metalloproteinase-12) ELISA Kit is a specialized assay designed for the precise measurement of MMP-12 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results that are essential for various research applications.MMP-12, also known as macrophage elastase, is a key enzyme involved in tissue remodeling and inflammation. It plays a crucial role in processes such as extracellular matrix degradation, cell migration, and immune response modulation.
Dysregulation of MMP-12 has been implicated in various diseases including chronic obstructive pulmonary disease (COPD), asthma, and atherosclerosis, highlighting its significance as a potential biomarker for studying and developing therapies for these conditions.Overall, the Human MMP-12 ELISA Kit offers researchers a valuable tool for studying the role of MMP-12 in disease pathology and potential therapeutic interventions. Its reliability and accuracy make it an essential component for any laboratory conducting research in the field of matrix metalloproteinases.
Product Name:
Human MMP-12 (Matrix Metalloproteinase 12) ELISA Kit
SKU:
HUES01410
Target:
Human MMP-12 (Matrix Metalloproteinase 12)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-12. You can calculate the concentration of Human MMP-12 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-103
85-99
94-111
Average (%)
94
91
101
1:4
Range (%)
92-107
79-91
82-94
Average (%)
97
86
88
1:8
Range (%)
87-101
84-100
87-100
Average (%)
94
91
93
1:16
Range (%)
90-106
80-92
83-98
Average (%)
98
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
93-108
99
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.01
8.16
1.15
1.94
8.53
Standard deviation
0.06
0.11
0.41
0.07
0.11
0.31
C V (%)
5.5
5.47
5.02
6.09
5.67
3.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-12 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-12 in samples. No significant cross-reactivity or interference between Human MMP-12 and analogues was observed.
