Human MMP-13 (Matrix Metalloproteinase 13) CLIA Kit
The Human MMP-13 (Matrix Metalloproteinase-13) CLIA Kit is specifically designed for the quantitative detection of MMP-13 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MMP-13 is an important enzyme involved in the degradation of extracellular matrix proteins, playing a crucial role in tissue remodeling and repair processes. Dysregulation of MMP-13 has been linked to various diseases, including arthritis, cancer, and cardiovascular disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its reliable performance and ease of use, the Human MMP-13 CLIA Kit is an essential tool for researchers seeking to explore the role of MMP-13 in disease pathogenesis and development. Get your kit today and advance your research efforts in the field of matrix metalloproteinases.
Product Name:
Human MMP-13 (Matrix Metalloproteinase 13) CLIA Kit
SKU:
HUES00121
Target:
Human MMP-13 (Matrix Metalloproteinase 13)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-13. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-13. You can calculate the concentration of Human MMP-13 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
97-112
92-108
Average (%)
95
104
100
1:4
Range (%)
87-98
84-96
87-99
Average (%)
92
91
93
1:8
Range (%)
103-121
95-112
98-114
Average (%)
110
102
105
1:16
Range (%)
102-119
89-99
88-100
Average (%)
109
94
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
106
EDTA plasma (n=5)
89-104
95
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
248.29
763.9
2041.85
249.21
747.59
1876.96
Standard deviation
23.31
62.41
176.21
22.85
79.32
126.51
C V (%)
9.39
8.17
8.63
9.17
10.61
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-13 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-13 in samples. No significant cross-reactivity or interference between Human MMP-13 and analogues was observed.
