The Human MMP-7 (Matrix Metalloproteinase-7) CLIA Kit is specifically designed for the accurate measurement of MMP-7 levels in human biological samples such as serum and plasma. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.MMP-7, also known as matrilysin, is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in tissue remodeling and repair processes.
Dysregulation of MMP-7 has been implicated in various diseases, including cancer and inflammatory conditions, making it a valuable biomarker for understanding disease progression and potential therapeutic targets.With its reliable performance and ease of use, the Human MMP-7 CLIA Kit is an invaluable tool for researchers and clinicians looking to explore the role of MMP-7 in health and disease.
Product Name:
Human MMP-7 (Matrix Metalloproteinase 7) CLIA Kit
SKU:
HUES00826
Target:
Human MMP-7 (Matrix Metalloproteinase 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-7. You can calculate the concentration of Human MMP-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-101
98-112
92-107
Average (%)
93
105
98
1:4
Range (%)
88-100
95-106
95-109
Average (%)
93
100
102
1:8
Range (%)
93-107
89-103
89-101
Average (%)
101
94
95
1:16
Range (%)
87-101
88-100
89-102
Average (%)
94
95
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
105
EDTA plasma (n=5)
96-111
101
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.25
234.86
780.93
101.74
248.78
729.8
Standard deviation
11.4
20.01
80.98
11.16
20.9
66.56
C V (%)
10.53
8.52
10.37
10.97
8.4
9.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-7 in samples. No significant cross-reactivity or interference between Human MMP-7 and analogues was observed.
