Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit
The Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit is a reliable and accurate tool for measuring MMP-7 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for various research applications. MMP-7, also known as matrilysin, is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling and cell migration. Elevated levels of MMP-7 have been associated with various diseases including cancer, inflammatory conditions, and cardiovascular disorders, making it a valuable biomarker for studying disease progression and potential therapeutic interventions. With the Human MMP-7 ELISA Kit, researchers can accurately measure MMP-7 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. This kit is a valuable tool for researchers working in the fields of cancer biology, inflammation, and tissue remodeling.
Product Name:
Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit
SKU:
HUES02484
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.