The Human MMP-9 (Matrix Metalloproteinase-9) CLIA Kit is a highly sensitive and specific assay designed for the precise quantification of MMP-9 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it an ideal tool for a wide range of research applications.MMP-9 is a key enzyme involved in extracellular matrix remodeling, cell migration, and tissue repair processes. It plays a crucial role in various physiological and pathological conditions such as inflammation, wound healing, cancer metastasis, and cardiovascular diseases.
Quantifying MMP-9 levels can provide valuable insight into disease progression and prognosis, making it a valuable biomarker for research and potential therapeutic targeting.Overall, the Human MMP-9 CLIA Kit offers researchers a reliable and efficient method for studying MMP-9 expression and activity, enabling a better understanding of its role in health and disease.
Product Name:
Human MMP-9 (Matrix Metalloproteinase 9) CLIA Kit
SKU:
HUES00828
Target:
Human MMP-9 (Matrix Metalloproteinase 9)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP-9. You can calculate the concentration of Human MMP-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
101-116
89-104
Average (%)
105
108
95
1:4
Range (%)
96-110
95-108
89-106
Average (%)
102
103
97
1:8
Range (%)
91-103
87-100
86-97
Average (%)
97
92
91
1:16
Range (%)
93-106
96-112
91-104
Average (%)
98
104
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
104
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
102-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.89
625.04
1419.98
223.42
687.02
1340.57
Standard deviation
20.76
47.38
127.09
23.59
74.54
142.37
C V (%)
9.4
7.58
8.95
10.56
10.85
10.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-9 in samples. No significant cross-reactivity or interference between Human MMP-9 and analogues was observed.
