The E2 (Estradiol) ELISA Kit is designed for the quantitative detection of Estradiol levels in various biological samples from humans, monkeys, and mice. Estradiol is a crucial estrogen hormone that plays a significant role in a wide array of physiological activities, such as cell growth, differentiation, and metabolic homeostasis. It influences various biological systems including the reproductive system, bone structure, cardiovascular system, and central nervous system. Altered levels of Estradiol have been implicated in various conditions like infertility, bone disorders, cardiovascular diseases, and certain cancers.
Therefore, accurate measurement of Estradiol is essential for understanding its role in these mechanisms and developing targeted therapeutic strategies. Assay Genie's E2 (Estradiol) ELISA Kit offers exceptional sensitivity and accuracy, ensuring reliable and reproducible results. Manufactured under strict quality control standards, this kit provides robust performance and is straightforward to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's E2 (Estradiol) ELISA Kit for the precise and dependable quantification of this key biomarker in your studies.
Product Name:
Human/Monkey/Mouse E2 (Estradiol) ELISA Kit
SKU:
AEES00011
Target:
Human/Monkey/Mouse E2 (Estradiol)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
5.63 pg/mL
Detection range:
9.37-600 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. During the reaction, E2 in the sample or standard competes with a fixed amount of E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to E2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-91
88-102
85-108
Average (%)
85
90
93
1:4
Range (%)
80-96
87-95
80-112
Average (%)
89
89
92
1:8
Range (%)
89-101
84-89
83-96
Average (%)
92
86
87
1:16
Range (%)
87-105
87-115
84-96
Average (%)
91
98
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
80-95
84
EDTA plasma (n=5)
82-98
87
Cell culture media (n=5)
85-112
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.55
100.44
548.02
22.84
105.23
545.22
Standard deviation
1.71
6.57
28.88
2.11
8.39
44.87
C V (%)
8.33
6.55
5.27
9.27
7.98
8.23
Sample type &Sample volume:
serum, plasma, urine, saliva; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum, plasma, urine, saliva.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
