Human MRP1 (Multidrug Resistance Associated Protein 1) CLIA Kit
The Human MRP1 (Multidrug Resistance-Associated Protein 1) CLIA Kit is specifically designed for the accurate measurement of MRP1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research purposes.MRP1 is a vital protein involved in multidrug resistance, playing a key role in drug efflux and resistance to chemotherapy agents.
Its overexpression has been linked to various diseases, including cancer, making it an important biomarker for studying drug resistance mechanisms and developing targeted therapies.Overall, the Human MRP1 CLIA Kit is an essential tool for researchers investigating drug resistance and exploring potential strategies to overcome this challenge in the treatment of various diseases.
Product Name:
Human MRP1 (Multidrug Resistance Associated Protein 1) CLIA Kit
SKU:
HUES01088
Target:
Human MRP1 (Multidrug Resistance Associated Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MRP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MRP1. You can calculate the concentration of Human MRP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
101-115
84-98
Average (%)
94
109
90
1:4
Range (%)
89-103
100-114
97-113
Average (%)
95
107
104
1:8
Range (%)
99-111
97-109
98-111
Average (%)
104
102
105
1:16
Range (%)
92-105
95-110
85-97
Average (%)
99
101
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-107
100
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200.99
449.13
1771.68
219.19
485.85
1899.14
Standard deviation
20.6
35.98
181.95
18.46
40.08
179.85
C V (%)
10.25
8.01
10.27
8.42
8.25
9.47
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MRP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MRP1 in samples. No significant cross-reactivity or interference between Human MRP1 and analogues was observed.
