The Human MT (Melatonin) ELISA Kit is specifically designed for the quantitative detection of melatonin levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Melatonin is a hormone produced by the pineal gland that regulates the sleep-wake cycle and has antioxidant properties. It plays a critical role in maintaining circadian rhythm and promoting healthy sleep patterns. Abnormal melatonin levels have been linked to sleep disorders, mood disorders, and other health conditions, making it a valuable biomarker for studying these conditions and developing potential treatments.
This ELISA kit provides a reliable tool for researchers to investigate the physiological functions and clinical implications of melatonin in health and disease. Its easy-to-use format and high-performance characteristics make it an essential asset for any laboratory conducting melatonin-related research.
Product Name:
Human MT (Melatonin) ELISA Kit
SKU:
HUES02946
Target:
Human MT (Melatonin)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
87-100
88-100
Average (%)
98
92
94
1:4
Range (%)
90-105
88-101
95-110
Average (%)
96
94
101
1:8
Range (%)
85-96
92-105
97-110
Average (%)
90
98
103
1:16
Range (%)
84-95
91-103
95-109
Average (%)
90
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
92-106
98
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.9
151.7
400.2
43.5
161.2
418.6
Standard deviation
2.5
7.9
20.8
2.3
6.9
23.0
C V (%)
5.45
5.21
5.2
5.29
4.28
5.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MT in samples. No significant cross-reactivity or interference between MT and analogues was observed.
