The Human MUC1 (Mucin 1) ELISA Kit is specifically designed for the precise measurement of Mucin 1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Mucin 1, also known as MUC1, is a glycoprotein that plays a critical role in cell signaling, adhesion, and immune response regulation.
Abnormal Mucin 1 expression has been associated with various diseases, including cancer, inflammatory disorders, and infectious diseases. Therefore, the Human MUC1 ELISA Kit serves as a valuable tool for studying Mucin 1-related pathologies and developing potential therapeutic strategies.
Product Name:
Human MUC1 (Mucin 1) ELISA Kit
SKU:
HUES01780
Target:
Human MUC1 (Mucin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC1. You can calculate the concentration of Human MUC1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
97-111
91-106
Average (%)
102
104
99
1:4
Range (%)
93-111
84-97
91-105
Average (%)
101
90
97
1:8
Range (%)
96-112
82-92
92-105
Average (%)
104
87
98
1:16
Range (%)
96-110
86-100
95-109
Average (%)
103
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
95-109
100
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.5
4.4
0.5
1.5
4.7
Standard deviation
0.03
0.07
0.18
0.03
0.06
0.17
C V (%)
6
4.67
4.09
6.0
4.0
3.62
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MUC1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC1 in samples. No significant cross-reactivity or interference between Human MUC1 and analogues was observed.
