The Human MUC2 (Mucin 2) CLIA Kit is specifically designed for the precise measurement of MUC2 levels in human samples, including serum, plasma, and cell culture supernatants. This CLIA kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.MUC2 is a critical mucin protein that plays a vital role in mucosal protection and barrier function in the human body. Dysregulation of MUC2 expression has been linked to various gastrointestinal diseases, including inflammatory bowel disease and colorectal cancer.
As a key biomarker in these conditions, studying MUC2 levels can provide valuable insights into disease progression and potential therapeutic targets.With its advanced technology and proven performance, the Human MUC2 CLIA Kit is an essential tool for researchers and clinicians seeking to understand the role of MUC2 in health and disease. Trust in this reliable assay to streamline your investigations and drive meaningful discoveries in the field of mucosal biology.
Product Name:
Human MUC2 (Mucin 2) CLIA Kit
SKU:
HUES00418
Target:
Human MUC2 (Mucin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC2. You can calculate the concentration of Human MUC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-96
92-106
102-117
Average (%)
90
99
110
1:4
Range (%)
93-107
88-100
93-104
Average (%)
98
93
99
1:8
Range (%)
94-109
85-97
87-99
Average (%)
99
91
92
1:16
Range (%)
93-108
102-117
94-110
Average (%)
98
109
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
100-116
106
Cell culture media (n=5)
90-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
233.58
686.27
2086.93
247.44
716.75
1996.72
Standard deviation
22.87
69.93
232.07
29.2
56.34
147.16
C V (%)
9.79
10.19
11.12
11.8
7.86
7.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC2 in samples. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.
