The Human MUC5AC (Mucin 5 Subtype Ac) CLIA Kit is a powerful tool for the accurate measurement of MUC5AC levels in human samples such as serum, plasma, and tissue culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.MUC5AC is a key mucin protein that plays a critical role in protecting the respiratory tract against pathogens and maintaining mucosal homeostasis. Abnormalities in MUC5AC expression have been linked to conditions such as asthma, chronic obstructive pulmonary disease (COPD), and cystic fibrosis, highlighting its importance as a potential biomarker for respiratory diseases.
By utilizing the Human MUC5AC CLIA Kit, researchers can gain valuable insights into the role of MUC5AC in respiratory health and disease, paving the way for advancements in diagnosis, treatment, and therapeutic development in this field.
Product Name:
Human MUC5AC (Mucin 5 Subtype AC) CLIA Kit
SKU:
HUES01187
Target:
Human MUC5AC (Mucin 5 Subtype AC)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC5AC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC5AC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC5AC. You can calculate the concentration of Human MUC5AC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-116
88-98
96-108
Average (%)
106
93
102
1:4
Range (%)
96-110
99-116
102-114
Average (%)
104
106
108
1:8
Range (%)
97-112
101-116
83-96
Average (%)
104
106
90
1:16
Range (%)
97-109
104-120
95-110
Average (%)
103
110
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-114
104
EDTA plasma (n=5)
97-110
105
Cell culture media (n=5)
100-114
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.62
107.89
352.39
52.64
114.8
336.15
Standard deviation
6.4
9.89
31.5
6.47
10.39
21.04
C V (%)
11.94
9.17
8.94
12.29
9.05
6.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC5AC concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC5AC in samples. No significant cross-reactivity or interference between Human MUC5AC and analogues was observed.
