Human Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit
The Human Myosin Binding Protein C Cardiac (MyBPC3) ELISA Kit is a cutting-edge tool for the quantitative measurement of MyBPC3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable asset for a variety of research studies.MyBPC3 is a critical protein involved in cardiac muscle function, playing a key role in regulating muscle contraction and relaxation. Dysregulation of MyBPC3 has been linked to various cardiac disorders, including hypertrophic cardiomyopathy.
By accurately measuring MyBPC3 levels, researchers can gain valuable insights into the underlying mechanisms of heart diseases and explore potential therapeutic interventions.Whether investigating cardiovascular conditions, studying muscle physiology, or developing novel treatments, the Human Myosin Binding Protein C Cardiac (MyBPC3) ELISA Kit provides a reliable and precise method for analyzing MyBPC3 expression levels. Trust in this advanced kit to elevate your research efforts and pave the way for new discoveries in the field of cardiology.
Product Name:
Human Myosin Binding Protein C, Cardiac (MYBPC3) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
