SynonymsEAT,MCL1L,MCL1S,BCL2L3,TM,Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1,Bcl-2-like protein 3,Bcl-2-related protein EAT/mcl1,mcl1/EAT
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL01811
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07820
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
EAT,MCL1L,MCL1S,BCL2L3,TM,Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1,Bcl-2-like protein 3,Bcl-2-related protein EAT/mcl1,mcl1/EAT
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit
The Human Myeloid Cell Leukemia Sequence 1 (MCL1) ELISA Kit from Assay Genie is a powerful tool for detecting levels of the MCL1 protein in human samples. MCL1 is a key player in cell survival and is often overexpressed in cancer cells, making it a promising target for cancer therapy research.This ELISA kit offers high sensitivity and specificity, ensuring accurate and reliable results in serum, plasma, and cell culture supernatants.
Whether studying leukemia, lymphoma, or other diseases where MCL1 is implicated, this kit provides a valuable tool for researchers looking to understand the role of this protein in disease progression.Trust Assay Genie's Human MCL1 ELISA Kit for precise detection of MCL1 levels in your samples, and unlock new insights into the mechanisms underlying cancer and other conditions related to MCL1 expression.
Product Name:
Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit
Product Code:
HUDL01811
Size:
96 Assays
Target:
MCL1
Synonyms:
EAT, MCL1L, MCL1S, BCL2L3, TM, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
