Human N-Acetyltransferase 8 Like Protein (NAT8L) ELISA Kit
The Human N-Acetyltransferase 8-Like Protein (NAT8L) ELISA Kit is specially designed for the accurate measurement of NAT8L levels in human serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.NAT8L is a key protein involved in various cellular processes, including metabolic regulation and neurotransmitter synthesis. Dysregulation of NAT8L has been linked to neurological disorders, metabolic diseases, and kidney disorders, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human NAT8L ELISA Kit, researchers can easily quantify NAT8L levels in biological samples, advancing their understanding of the protein's role in health and disease. Trust in this innovative kit for reliable and accurate results in your research endeavors.
Product Name:
Human N-Acetyltransferase 8 Like Protein (NAT8L) ELISA Kit
Product Code:
HUDL01989
Size:
96 Assays
Target:
NAT8L
Synonyms:
Hcml3, CML3, NAA synthetase, N-acetylaspartate synthetase, Camello-like protein 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
