The Human NKCC2 (Na-K-Cl Cotransporter 2) ELISA Kit is specially designed for the precise measurement of NKCC2 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable tool for various research applications.NKCC2 is a vital transporter protein involved in ion transport across cell membranes, playing a crucial role in electrolyte balance and fluid homeostasis.
Dysregulation of NKCC2 function has been linked to various renal disorders, making it a key target for understanding and treating renal diseases.By utilizing the Human NKCC2 ELISA Kit, researchers can gain valuable insights into the role of NKCC2 in health and disease, paving the way for potential therapeutic interventions targeting this important transporter protein.
Product Name:
Human Na-K-Cl Cotransporter 2 (NKCC2) ELISA Kit
Product Code:
HUDL02044
Size:
96 Assays
Target:
NKCC2
Synonyms:
SLC12A1, NKCC2, Solute Carrier Family 12 Member 1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
