The Human NCAD (Neural Cadherin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NCAD levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Neural Cadherin, also known as NCAD, is a key protein involved in cell adhesion and cell signaling, playing a critical role in neuronal development and function.
Dysregulation of NCAD has been linked to various neurological disorders, including Alzheimer's disease and schizophrenia. Therefore, measuring NCAD levels can provide valuable insights into these conditions and potential therapeutic targets.The Human NCAD CLIA Kit from AssayGenie offers researchers a powerful tool to investigate the role of NCAD in health and disease, contributing to advancements in neuroscience research and the development of novel treatments.
Product Name:
Human NCAD (Neural Cadherin) CLIA Kit
SKU:
HUES00168
Target:
Human NCAD (Neural Cadherin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NCAD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NCAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NCAD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NCAD. You can calculate the concentration of Human NCAD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
101-114
100-117
Average (%)
92
108
108
1:4
Range (%)
88-101
93-106
96-110
Average (%)
94
100
102
1:8
Range (%)
84-95
95-109
100-116
Average (%)
90
102
108
1:16
Range (%)
102-118
90-104
100-117
Average (%)
109
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-118
108
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
95-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.28
304.93
909.53
107.06
299.9
886.09
Standard deviation
10.67
22.38
79.22
9.12
32.84
84.98
C V (%)
9.85
7.34
8.71
8.52
10.95
9.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NCAD concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NCAD in samples. No significant cross-reactivity or interference between Human NCAD and analogues was observed.
