The Human NCAD (Neural Cadherin) ELISA Kit is specifically designed for the precise quantification of neural cadherin levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes. Neural cadherin, also known as N-cadherin, is a key protein involved in cell-cell adhesion and neuronal migration. It plays a critical role in neural development and function, making it a valuable biomarker for studying neurodevelopmental disorders, neurodegenerative diseases, and cancer metastasis.
With this ELISA kit, researchers can easily measure neural cadherin levels in human samples, providing valuable insights into the role of this protein in various physiological and pathological processes. Just like the Human ANG ELISA Kit, this product is a powerful tool for investigating neural cadherin biology and developing potential therapeutic interventions.
Product Name:
Human NCAD (Neural Cadherin) ELISA Kit
SKU:
HUES01461
Target:
Human NCAD (Neural Cadherin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NCAD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NCAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NCAD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NCAD. You can calculate the concentration of Human NCAD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
94-106
93-109
Average (%)
91
100
100
1:4
Range (%)
88-104
80-93
85-99
Average (%)
96
87
92
1:8
Range (%)
95-110
85-97
87-100
Average (%)
100
92
92
1:16
Range (%)
90-103
85-97
85-97
Average (%)
95
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
0.94
4.98
0.56
0.92
5.14
Standard deviation
0.03
0.05
0.25
0.04
0.04
0.27
C V (%)
5.77
5.32
5.02
7.14
4.35
5.25
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NCAD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NCAD in samples. No significant cross-reactivity or interference between Human NCAD and analogues was observed.
