Human Neural Cell Adhesion Molecule (NCAM) ELISA Kit
The Human Neural Cell Adhesion Molecule (NCAM) ELISA Kit is specifically designed for the precise measurement of NCAM levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.NCAM is a key molecule involved in cell adhesion and neural development, playing a crucial role in processes such as cell migration, neurite outgrowth, and synaptic plasticity. Dysregulation of NCAM expression has been linked to various neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Human NCAM ELISA Kit is an essential tool for researchers studying neural development, neurodegenerative diseases, and other related fields. It provides a comprehensive solution for measuring NCAM levels with precision and confidence, contributing to the advancement of scientific knowledge and discovery.
Product Name:
Human Neural Cell Adhesion Molecule (NCAM) ELISA Kit
Product Code:
HUDL01992
Size:
96 Assays
Target:
NCAM
Synonyms:
CD56, NCAM1, MSK39, N-CAM-1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
