Human NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
The Human NGAL (Neutrophil Gelatinase-Associated Lipocalin) CLIA Kit is specifically designed for the precise detection of NGAL levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for various research purposes.NGAL is a key protein associated with neutrophil activation and inflammation, serving as a biomarker for kidney injury, sepsis, and various other diseases.
Its role in identifying and monitoring such conditions makes it a valuable tool for research and diagnostic applications. With the Human NGAL CLIA Kit, researchers can confidently measure NGAL levels and gain valuable insights into the mechanisms underlying various diseases, ultimately leading to advancements in treatment and patient care.
Product Name:
Human NGAL (Neutrophil Gelatinase Associated Lipocalin) CLIA Kit
SKU:
HUES00092
Target:
Human NGAL (Neutrophil Gelatinase Associated Lipocalin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
99-113
96-113
Average (%)
97
107
103
1:4
Range (%)
102-115
93-106
86-100
Average (%)
109
100
93
1:8
Range (%)
95-111
87-103
99-114
Average (%)
102
94
105
1:16
Range (%)
87-98
92-104
86-98
Average (%)
92
97
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-109
104
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.8
190.47
894.54
92.78
207.9
905.47
Standard deviation
10.15
19.12
67.63
11.66
18.86
81.22
C V (%)
10.07
10.04
7.56
12.57
9.07
8.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGAL in samples. No significant cross-reactivity or interference between Human NGAL and analogues was observed.
