Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
The Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit is thoughtfully engineered for the precise and quantitative detection of Neutrophil Gelatinase Associated Lipocalin in diverse human biological samples. NGAL is a crucial biomarker involved in various pathological conditions, particularly in acute kidney injury and chronic kidney disease, making it indispensable for monitoring renal function and identifying kidney-related issues at an early stage. Our ELISA kit is distinguished by its remarkable sensitivity and specificity, which guarantee accurate and reproducible results critical for renal health research.
With a focus on stringent quality control standards, this kit delivers robust and consistent performance and is designed to be user-friendly, ensuring an excellent choice for your research needs. Trust in Assay Genie's NGAL ELISA Kit for reliable and precise quantification of this pivotal biomarker in your scientific investigations.
Product Name:
Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
SKU:
AEES00155
Target:
Human NGAL (Neutrophil Gelatinase Associated Lipocalin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGAL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
95-107
87-101
Average (%)
97
101
94
1:4
Range (%)
89-105
99-113
85-95
Average (%)
96
104
90
1:8
Range (%)
88-101
97-111
90-105
Average (%)
95
104
97
1:16
Range (%)
88-101
96-112
90-102
Average (%)
95
103
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.11
250.16
953.57
107.72
251.7
944.02
Standard deviation
5.69
10.06
45.01
6.16
11.68
51.45
C V (%)
5.8
4.02
4.72
5.72
4.64
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGAL in samples. No significant cross-reactivity or interference between Human NGAL and analogues was observed.
