The Human NGF (Nerve Growth Factor) CLIA Kit is specifically designed for the precise measurement of NGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.NGF is a key protein that plays a vital role in nerve cell development, growth, and maintenance. It is essential for the functioning of the nervous system and is implicated in various neurological conditions, including Alzheimer's disease, peripheral neuropathy, and neurotrophic keratitis.
As such, NGF serves as a critical biomarker for studying these disorders and potentially developing novel treatments.The Human NGF CLIA Kit from AssayGenie provides researchers with a reliable tool to quantify NGF levels in human samples, enabling deeper insights into the role of this important protein in health and disease.
Product Name:
Human NGF (Nerve Growth Factor) CLIA Kit
SKU:
HUES00689
Target:
Human NGF (Nerve Growth Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
101-117
96-109
Average (%)
98
109
101
1:4
Range (%)
102-114
99-112
88-104
Average (%)
108
106
95
1:8
Range (%)
91-106
87-98
84-97
Average (%)
97
93
90
1:16
Range (%)
97-113
96-110
92-107
Average (%)
105
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-107
102
EDTA plasma (n=5)
100-113
106
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.37
45.86
175.03
23.02
43.15
181.52
Standard deviation
2.23
5.17
10.75
2.4
5.01
13.21
C V (%)
9.15
11.27
6.14
10.43
11.61
7.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
